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  "author": "Patricia Sobecky",
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  "author": "Patricia Sobecky",
            
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  "author_email": "psobecky@ua.edu",
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  "author_email": "psobecky@ua.edu",
            
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      "key": "DOI",
            
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      "value": "doi:10.7266/N7BP017Z"
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      "value": "doi:10.7266/N7BP017Z"
            
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      "key": "ISO.pointOfContact",
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      "key": "ISO.pointOfContact",
            
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      "value": "Patricia Sobecky <psobecky@ua.edu>"
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      "value": "Patricia Sobecky <psobecky@ua.edu>"
            
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    },
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    {
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    {
            
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      "key": "Place Keywords",
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      "key": "Place Keywords",
            
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      "value": "Chandeleur Islands, Red Fish Point, Gulf of Mexico, 
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      "value": "Chandeleur Islands, Red Fish Point, Gulf of Mexico, 
            
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Louisiana"
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Louisiana"
            
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    },
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    },
            
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    {
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    {
            
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      "key": "Temporal Begin",
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      "key": "Temporal Begin",
            
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      "value": "2015-07-09"
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      "value": "2015-07-09"
            
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      "key": "Temporal End",
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      "key": "Temporal End",
            
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      "value": "2015-09-09"
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      "value": "2015-09-09"
            
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    },
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      "key": "Theme Keywords",
            
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      "value": "nitrogen cycling, biodiversity, microbial ecology, 
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      "value": "nitrogen cycling, biodiversity, microbial ecology, 
            
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Deepwater Horizon, metagenomics"
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Deepwater Horizon, metagenomics"
            
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  "metadata_modified": "2022-07-27T14:33:39.557771",
            
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  "name": 
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  "name": 
            
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l-community-composition-within-chandeleur-island-louisiana-sn7bp017z",
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l-community-composition-within-chandeleur-island-louisiana-sn7bp017z",
            
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  "notes": "### Abstract\nOil spills such as the Deepwater Horizon 
50
  "notes": "### Abstract\nOil spills such as the Deepwater Horizon 
            
51
(DWH) disaster can significantly impact biochemical and biological 
51
(DWH) disaster can significantly impact biochemical and biological 
            
52
processes in coastal salt marsh sediments imparting short-term and 
52
processes in coastal salt marsh sediments imparting short-term and 
            
53
long-term effects on ecosystem functions. We sought to identify 
53
long-term effects on ecosystem functions. We sought to identify 
            
54
functional and biogeochemical factors important for driving 
54
functional and biogeochemical factors important for driving 
            
55
denitrification in Chandeleur saltmarsh habitats. Denitrification, a 
55
denitrification in Chandeleur saltmarsh habitats. Denitrification, a 
            
56
biogeochemical process, is a vital ecosystem service providing 
56
biogeochemical process, is a vital ecosystem service providing 
            
57
considerable ecological and economic value in coastal habitats, 
57
considerable ecological and economic value in coastal habitats, 
            
58
particularly in salt marsh ecosystems. Studies focused on the 
58
particularly in salt marsh ecosystems. Studies focused on the 
            
59
long-term impact of heavy oiling on microbial populations carrying out 
59
long-term impact of heavy oiling on microbial populations carrying out 
            
60
denitrification in northern Gulf of Mexico (nGoM) marsh ecosystems are 
60
denitrification in northern Gulf of Mexico (nGoM) marsh ecosystems are 
            
61
surprisingly limited. Using sediment samples from Red Fish Point 
61
surprisingly limited. Using sediment samples from Red Fish Point 
            
62
measured for N cycle flux during Year 1 studies, we embarked on a deep 
62
measured for N cycle flux during Year 1 studies, we embarked on a deep 
            
63
metagenomic survey (microbial structure and function) of Chandeleur 
63
metagenomic survey (microbial structure and function) of Chandeleur 
            
64
Islands (Louisiana) habitats for the purpose of characterizing extant 
64
Islands (Louisiana) habitats for the purpose of characterizing extant 
            
65
microbial community composition. The specific objectives of this study 
65
microbial community composition. The specific objectives of this study 
            
66
were to: i) determine if temporal (July-September 2015) changes occur 
66
were to: i) determine if temporal (July-September 2015) changes occur 
            
67
for known denitrifying taxonomic groups, ii) determine the 
67
for known denitrifying taxonomic groups, ii) determine the 
            
68
contributions of N cycle flux measurements relative to microbial 
68
contributions of N cycle flux measurements relative to microbial 
            
69
community composition, and iii) characterize putative functional 
69
community composition, and iii) characterize putative functional 
            
70
potential for nitrogen cycling and sulfur oxidation pathways. Analysis 
70
potential for nitrogen cycling and sulfur oxidation pathways. Analysis 
            
71
of ~1.3 Terabytes of sequences derived from 30 different vegetated and 
71
of ~1.3 Terabytes of sequences derived from 30 different vegetated and 
            
72
subtidal marsh sediment samples are discussed. Data highlight salt 
72
subtidal marsh sediment samples are discussed. Data highlight salt 
            
73
marsh functional and taxonomic biodiversity within a previously oiled 
73
marsh functional and taxonomic biodiversity within a previously oiled 
            
74
marsh ecosystem. The combination of molecular ecology methods to 
74
marsh ecosystem. The combination of molecular ecology methods to 
            
75
assess microbial biodiversity coupled with biogeochemical measurements 
75
assess microbial biodiversity coupled with biogeochemical measurements 
            
76
provides greater insights into marsh microbial interactions and N 
76
provides greater insights into marsh microbial interactions and N 
            
77
cycling five years after the 2010 DWH spill.\n\n### Purpose\nThe 
77
cycling five years after the 2010 DWH spill.\n\n### Purpose\nThe 
            
78
purpose of this study was to i) incorporate a deep metagenomic 
78
purpose of this study was to i) incorporate a deep metagenomic 
            
79
approach to temporally profile microbial community composition and 
79
approach to temporally profile microbial community composition and 
            
80
functional potential ii) characterize putative salt marsh ecosystem 
80
functional potential ii) characterize putative salt marsh ecosystem 
            
81
function (i.e. denitrification, nitrogen fixation, sulfur oxidation) 
81
function (i.e. denitrification, nitrogen fixation, sulfur oxidation) 
            
82
between habitat types (i.e. marsh, subtidal) five years following the 
82
between habitat types (i.e. marsh, subtidal) five years following the 
            
83
DWH oil spill and iii) characterize correlations between 
83
DWH oil spill and iii) characterize correlations between 
            
84
biogeochemical measurements and metagenomic taxonomic 
84
biogeochemical measurements and metagenomic taxonomic 
            
85
profiles.\n\n**DOI: 
85
profiles.\n\n**DOI: 
            
86
[doi:10.7266/N7BP017Z](https://www.doi.org/10.7266/N7BP017Z)**\n\n### 
86
[doi:10.7266/N7BP017Z](https://www.doi.org/10.7266/N7BP017Z)**\n\n### 
            
87
Suggested Citation\nNikaela Y. Flournoy. 2018. Metagenomic insights on 
87
Suggested Citation\nNikaela Y. Flournoy. 2018. Metagenomic insights on 
            
88
Microbial Community Composition within Chandeleur Island Louisiana 
88
Microbial Community Composition within Chandeleur Island Louisiana 
            
89
Salt Marsh Ecosystems, July \u2013 September, 2015. Distributed by: 
89
Salt Marsh Ecosystems, July \u2013 September, 2015. Distributed by: 
            
90
Gulf of Mexico Research Initiative Information and Data Cooperative 
90
Gulf of Mexico Research Initiative Information and Data Cooperative 
            
91
(GRIIDC), Harte Research Institute, Texas A&M University\u2013Corpus 
91
(GRIIDC), Harte Research Institute, Texas A&M University\u2013Corpus 
            
92
Christi. doi:10.7266/N7BP017Z\n\n**Funded by:** Gulf of Mexico 
92
Christi. doi:10.7266/N7BP017Z\n\n**Funded by:** Gulf of Mexico 
            
93
Research Initiative (GoMRI)\n\n**Funding cycle:** RFP-IV\n\n**Research 
93
Research Initiative (GoMRI)\n\n**Funding cycle:** RFP-IV\n\n**Research 
            
94
group:** Alabama Center for Ecological Resilience (ACER)\n\n",
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group:** Alabama Center for Ecological Resilience (ACER)\n\n",
            
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      "id": "1187d400-d9de-4166-aca2-2e591ecf8c65",
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      "id": "1187d400-d9de-4166-aca2-2e591ecf8c65",
            
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      "name": "Chandeleur Islands",
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      "name": "Chandeleur Islands",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Deepwater Horizon",
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      "display_name": "Deepwater Horizon",
            
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      "id": "0e13401a-3910-4b7f-9e17-df754fe3f24a",
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      "id": "0e13401a-3910-4b7f-9e17-df754fe3f24a",
            
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      "name": "Deepwater Horizon",
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      "name": "Deepwater Horizon",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
203
    {
            
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      "display_name": "Gulf of Mexico",
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      "display_name": "Gulf of Mexico",
            
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      "id": "26b2a6bc-e01b-4c22-9d3f-5bbcd148cc4f",
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      "id": "26b2a6bc-e01b-4c22-9d3f-5bbcd148cc4f",
            
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      "name": "Gulf of Mexico",
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      "name": "Gulf of Mexico",
            
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      "state": "active",
207
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
210
    {
            
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      "display_name": "Louisiana",
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      "display_name": "Louisiana",
            
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      "id": "d7699c97-9202-4624-bb36-265d2a628a57",
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      "id": "d7699c97-9202-4624-bb36-265d2a628a57",
            
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      "name": "Louisiana",
213
      "name": "Louisiana",
            
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      "state": "active",
214
      "state": "active",
            
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      "vocabulary_id": null
215
      "vocabulary_id": null
            
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    },
216
    },
            
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    {
217
    {
            
194
      "display_name": "Red Fish Point",
218
      "display_name": "Red Fish Point",
            
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      "id": "08d44b61-d616-4b27-ba1f-17381f754c34",
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      "id": "08d44b61-d616-4b27-ba1f-17381f754c34",
            
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      "name": "Red Fish Point",
220
      "name": "Red Fish Point",
            
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      "state": "active",
221
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
224
    {
            
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      "display_name": "biodiversity",
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      "display_name": "biodiversity",
            
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      "id": "16737851-e341-4f85-a496-dd82131afef6",
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      "id": "16737851-e341-4f85-a496-dd82131afef6",
            
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      "name": "biodiversity",
227
      "name": "biodiversity",
            
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      "state": "active",
228
      "state": "active",
            
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      "vocabulary_id": null
229
      "vocabulary_id": null
            
206
    },
230
    },
            
207
    {
231
    {
            
208
      "display_name": "metagenomics",
232
      "display_name": "metagenomics",
            
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      "id": "0084e987-f692-493e-8b7c-d145f821b369",
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      "id": "0084e987-f692-493e-8b7c-d145f821b369",
            
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      "name": "metagenomics",
234
      "name": "metagenomics",
            
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      "state": "active",
235
      "state": "active",
            
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      "vocabulary_id": null
236
      "vocabulary_id": null
            
213
    },
237
    },
            
214
    {
238
    {
            
215
      "display_name": "microbial ecology",
239
      "display_name": "microbial ecology",
            
216
      "id": "de5416a9-ab11-4a06-b707-f18878264fa0",
240
      "id": "de5416a9-ab11-4a06-b707-f18878264fa0",
            
217
      "name": "microbial ecology",
241
      "name": "microbial ecology",
            
218
      "state": "active",
242
      "state": "active",
            
219
      "vocabulary_id": null
243
      "vocabulary_id": null
            
220
    },
244
    },
            
221
    {
245
    {
            
222
      "display_name": "nitrogen cycling",
246
      "display_name": "nitrogen cycling",
            
223
      "id": "5dc58ca1-af9f-42fd-974a-8ab2649fe487",
247
      "id": "5dc58ca1-af9f-42fd-974a-8ab2649fe487",
            
224
      "name": "nitrogen cycling",
248
      "name": "nitrogen cycling",
            
225
      "state": "active",
249
      "state": "active",
            
226
      "vocabulary_id": null
250
      "vocabulary_id": null
            
227
    }
251
    }
            
228
  ],
252
  ],
            
229
  "title": "Metagenomic insights on Microbial Community Composition 
253
  "title": "Metagenomic insights on Microbial Community Composition 
            
230
within Chandeleur Island Louisiana Salt Marsh Ecosystems, July \u2013 
254
within Chandeleur Island Louisiana Salt Marsh Ecosystems, July \u2013 
            
231
September, 2015",
255
September, 2015",
            
232
  "type": "dataset",
256
  "type": "dataset",
            
233
  "url": null,
257
  "url": null,
            
234
  "version": null
258
  "version": null
            
235
}
259
}