Transect Sampling for Dissolved Gases, DMSP, and Bacterial Production (2010-2012)
Metadata:
- Identification_Information:
- Citation:
- Citation_Information:
- Originator:
- Dauphin Island Sea Lab: Ron Kiene Lab
- Publication_Date:
- Unpublished material
- Title:
- Transect Sampling for Dissolved Gases, DMSP, and Bacterial Production (2010-2012)
- Description:
- Abstract:
- Water samples were collected at bottom, mid-depth, and surface at six stations along a transect running from the middle of Mobile Bay to the shelf in the northern Gulf of Mexico, from June 2010 to May 2012. Data include concentrations of dimethyl sulfide (DMS), dimethylsulfoniopropionate (DMSP), dissolved oxygen, and dissolved methane, and bacterial biomass production via leucine incorporation. The transect utilized for this study is part of the Fisheries Oceanography of Coastal Alabama (FOCAL) program, which collects long-term baseline hydrographic data and plankton samples toward understanding the highly variable nature of Alabama's nearshore environment as it relates to fisheries production.
- Purpose:
- The purpose of this study was to understand carbon and oxygen dynamics on the Alabama shelf in relation to potential inputs of hydrocarbons from the BP-Macondo spill.
- Time_Period_of_Content:
- Time_Period_Information:
- Range_of_Dates/Times:
- Beginning_Date:
- 20100615
- Ending_Date:
- 20120515
- Currentness_Reference:
- ground condition
- Status:
- Progress:
- Complete
- Maintenance_and_Update_Frequency:
- As needed
- Spatial_Domain:
- Bounding_Coordinates:
- West_Bounding_Coordinate:
- -88.212
- East_Bounding_Coordinate:
- -88.01
- North_Bounding_Coordinate:
- 30.437
- South_Bounding_Coordinate:
- 29.79
- Keywords:
- Theme:
- Theme_Keyword_Thesaurus:
- ISO 19115 Topic Categories
- Theme_Keyword:
- environment
- Theme_Keyword:
- inlandWaters
- Theme_Keyword:
- oceans
- Theme:
- Theme_Keyword_Thesaurus:
- None
- Theme_Keyword:
- Dimethyl sulfide
- Theme_Keyword:
- DMS
- Theme_Keyword:
- Dimethylsulfoniopropionate
- Theme_Keyword:
- DMSP
- Theme_Keyword:
- Sulfur cycle
- Theme_Keyword:
- Dissolved oxygen
- Theme_Keyword:
- Dissolved methane
- Theme_Keyword:
- Dissolved gases
- Theme_Keyword:
- Water quality
- Theme_Keyword:
- Bacterial biomass production
- Theme_Keyword:
- Leucine incorporation
- Theme_Keyword:
- Temperature
- Theme_Keyword:
- Salinity
- Theme_Keyword:
- Water sampling
- Place:
- Place_Keyword_Thesaurus:
- None
- Place_Keyword:
- DISL
- Place_Keyword:
- Dauphin Island Sea Lab
- Place_Keyword:
- Mobile Bay
- Place_Keyword:
- Gulf of Mexico
- Place_Keyword:
- Alabama
- Access_Constraints:
- Permission to access these data must be given by Dr. Ron Kiene of the Dauphin Island Sea Lab.
- Use_Constraints:
- Acknowledgment of the Dauphin Island Sea Lab (DISL), Marine Environmental Sciences Consortium (MESC), Gulf of Mexico Research Initiative (GoMRI), British Petroleum (BP), the Fisheries Oceanography of Coastal Alabama (FOCAL) program, and the Alabama Department of Conservation and Natural Resources would be appreciated in products developed from these data, and such acknowledgment as is standard for citation and legal practices for data source is expected by users of these data. Users should be aware that comparison with other data sets for the same area from other time periods may be inaccurate due to inconsistencies resulting from changes in mapping conventions, data collection, and computer processes over time. The distributor shall not be liable for improper or incorrect use of these data, based on the description of appropriate/inappropriate uses described in the metadata document. These data are not legal documents and are not to be used as such.
- Point_of_Contact:
- Contact_Information:
- Contact_Person_Primary:
- Contact_Person:
- Ron Kiene
- Contact_Organization:
- Dauphin Island Sea Lab
- Contact_Position:
- Principal Investigator
- Contact_Address:
- Address_Type:
- mailing and physical
- Address:
- 101 Bienville Boulevard
- City:
- Dauphin Island
- State_or_Province:
- AL
- Postal_Code:
- 36528
- Contact_Voice_Telephone:
- 251-861-2141 x2269
- Contact_Electronic_Mail_Address:
- rkiene@disl.org
- Browse_Graphic:
- Browse_Graphic_File_Name:
- http://www.ncddc.noaa.gov/approved_recs/org/disl/RKiene/Lab_work/transect-gases/DMSP_browse_FOCAL-transect-fig1.JPG
- Browse_Graphic_File_Description:
- Map of the Alabama shelf and lower Mobile Bay, showing the stations of the FOCAL transect. CP is the location of a permanent mooring with time-series hydrographic data. Map is from Dzwonkowski, B., Park, K., Ha, H.K., Graham, W.M., Hernandez, F.J., and S.P. Powers (2011). "Hydrographic variability on a coastal shelf directly influenced by estuarine outflow." Continental Shelf Research 31: 939–950.
- Browse_Graphic_File_Type:
- JPEG
- Data_Set_Credit:
- DISL: Kiene Lab
- Native_Data_Set_Environment:
- Microsoft Excel
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- Data_Quality_Information:
- Logical_Consistency_Report:
- N/A
- Completeness_Report:
- Water samples were collected at bottom, mid-depth, and surface approximately once per month from June 15 2010 to May 15 2012 at 6 stations on the FOCAL transect running from the middle of Mobile Bay to the shelf in the northern Gulf of Mexico. Samples were collected with Niskin bottles on a CTD rosette. Intervals between sampling ranged from 2 to 6 weeks. Surface samples were always collected, but sometimes the mid-depth and occasionally the bottom samples were omitted. Most sampling events included all six stations.
- Lineage:
- Process_Step:
- Process_Description:
- Dimethyl sulfide (DMS) concentrations: DMS concentrations measured by pure-and-trap gas chromatography with flame photometric detection (FPD). Two to four ml of seawater were purged with helium and the DMS cryotrapped in Teflon tubing. DMS introduced into Chromosil 330 column and quantified with FPD. Standards generated from permeation system. Precision was generally 20%. (Kiene, R. P. (1993). Measurement of dimethylsulfide (DMS) and dimethylsulfoniopropionate in seawater and estimation of DMS turnover rates. In P. Kemp, B. Sherr, E. Sherr & J. Cole (Eds.), Handbook of methods in aquatic microbial ecology (pp. 601-610). New York: Lewis Publishers.)
- Process_Date:
- Unknown
- Process_Step:
- Process_Description:
- Total Dimethylsulfoniopropionate (DMSP) concentrations: Ten ml water samples were acidified with HCl to 1.5% final acid concentration to preserve DMSP. Subsamples were removed and treated with 5 M NaOH in sealed vials. The resulting DMS was quantified by GC-FPD. Precision was generally 10%. (Kiene, R. P. (1993). Measurement of dimethylsulfide (DMS) and dimethylsulfoniopropionate in seawater and estimation of DMS turnover rates. In P. Kemp, B. Sherr, E. Sherr & J. Cole (Eds.), Handbook of methods in aquatic microbial ecology (pp. 601-610). New York: Lewis Publishers.)
- Process_Date:
- Unknown
- Process_Step:
- Process_Description:
- Dissolved oxygen concentrations: Water samples were collected directly from Niskin bottles via tubing into 60 ml BOD bottles. They were immediately fixed with alkaline iodide, and manganese chloride for Winkler titration. The titration was carried out with a Mettler DL-25 autotitrator. Precision was > 5%. (Kiene, R. P., & Linn, L. J. (2000). Distribution and turnover of dissolved DMSP and its relationship with bacterial production in the Gulf of Mexico. Limnol. Oceanogr., 45, 849-861. Pomeroy, L. R., Sheldon, J. E., & Sheldon, W. M., Jr. (1994). Changes in bacterial numbers and leucine assimilation during estimations of microbial respiratory rates in seawater by the precision Winkler method. Appl. Environ. Microbiol., 60, 328-332.)
- Process_Date:
- Unknown
- Process_Step:
- Process_Description:
- Dissolved methane concentrations: Methane was quantified with a headspace equilibration technique and GC-FID detection. Water samples were filled without gas exchange into 160 ml serum bottles. Immediately after filling, bottles were sealed with a rubber stopper leaving no headspace. Samples were stored at in situ temperature until analysis in the shore laboratory. To each bottle, 4 ml of pure N2 gas was introduced with a glass barreled syringe and 22 gage needle, allowing water displacement. The bottle was shaken vigorously to equilibrate liquid and gas phases. The headspace was sampled for CH4 concentration by GC-FID and the original dissolved CH4 concentration was obtained from the total methane in the bottle, accounting for gas and liquid phases and the temperature and salinity for that sample. Precision was 5% or better.
- Process_Date:
- Unknown
- Process_Step:
- Process_Description:
- Bacterial biomass production via leucine incorporation: Bacterial production was measured by tritiated (3H) leucine incorporation into TCA-insoluble macromolecules. The microcentrifuge method was used. Triplicate live samples (2 ml) and one TCA-killed sample per depth were treated with 20 nM of 3H-Leucine. Incubation was for 1 h and samples were killed by addition of TCA. Samples were spun at 15,000 x g in a microcentrifuge and after several rinses, the pellet was resuspended in Ecolumn scintillation cocktail and the 3H radioactivity quantified. Rates are reported in picomolar Leucine per hour. Precision is generally 10%. (Smith, D. C., & Azam, F. (1992). A simple, economical method for measuring bacterial protein synthesis rates in sea water using 3H-Leucine. Marine Microbial Food Webs, 6, 107-114. )
- Process_Date:
- Unknown
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- Entity_and_Attribute_Information:
- Detailed_Description:
- Entity_Type:
- Entity_Type_Label:
- FOCAL Transect Data for Gases - DMSP
- Entity_Type_Definition:
- A data table containing concentrations of dimethyl sulfide (DMS), total dimethylsulfoniopropionate (DMSP), dissolved oxygen, and dissolved methane, and bacterial biomass production via leucine incorporation, as measured from water samples taken at bottom, mid-depth, and surface along a CTD transect from the middle of Mobile Bay to the shelf in the northern Gulf of Mexico. Temperature and salinity data are also included.
- Entity_Type_Definition_Source:
- DISL: Kiene Lab
- Attribute:
- Attribute_Label:
- Year
- Attribute_Definition:
- The year that the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 2010
- Range_Domain_Maximum:
- 2012
- Attribute:
- Attribute_Label:
- Month
- Attribute_Definition:
- The month that the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Unrepresentable_Domain:
- Valid values are the names of the months (January, February, etc.).
- Attribute:
- Attribute_Label:
- Num Month
- Attribute_Definition:
- The month that the water sample was collected, represented by number.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 1
- Range_Domain_Maximum:
- 12
- Attribute:
- Attribute_Label:
- Day
- Attribute_Definition:
- The day of month that the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 1
- Range_Domain_Maximum:
- 31
- Attribute:
- Attribute_Label:
- Date
- Attribute_Definition:
- The date that the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Unrepresentable_Domain:
- Date format is MM/DD/YYYY.
- Attribute:
- Attribute_Label:
- Julian Day
- Attribute_Definition:
- The day of year that the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 1
- Range_Domain_Maximum:
- 366
- Attribute:
- Attribute_Label:
- Station
- Attribute_Definition:
- The station on the FOCAL transect where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Enumerated_Domain:
- Enumerated_Domain_Value:
- MB
- Enumerated_Domain_Value_Definition:
- Transect station is located at latitude 30.4374 longitude -88.0113.
- Enumerated_Domain_Value_Definition_Source:
- DISL: FOCAL Laboratory
- Enumerated_Domain_Value:
- DI
- Enumerated_Domain_Value_Definition:
- Transect station is located at latitude 30.25345 longitude -88.04355.
- Enumerated_Domain_Value_Definition_Source:
- DISL: FOCAL Laboratory
- Enumerated_Domain_Value:
- T10
- Enumerated_Domain_Value_Definition:
- Transect station is located at latitude 30.16092 longitude -88.12288.
- Enumerated_Domain_Value_Definition_Source:
- DISL: FOCAL Laboratory
- Enumerated_Domain_Value:
- T20
- Enumerated_Domain_Value_Definition:
- Transect station is at latitude 30.09016667 longitude -88.21156667.
- Enumerated_Domain_Value_Definition_Source:
- DISL: FOCAL Laboratory
- Enumerated_Domain_Value:
- C3
- Enumerated_Domain_Value_Definition:
- Transect station is at latitude 29.9413 longitude -88.21156667.
- Enumerated_Domain_Value_Definition_Source:
- DISL: FOCAL Laboratory
- Enumerated_Domain_Value:
- T35
- Enumerated_Domain_Value_Definition:
- Transect station is at latitude 29.79887 longitude -88.20828.
- Enumerated_Domain_Value_Definition_Source:
- DISL: FOCAL Laboratory
- Attribute:
- Attribute_Label:
- Latitude
- Attribute_Definition:
- The latitude where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 29.79
- Range_Domain_Maximum:
- 30.4374
- Attribute_Units_of_Measure:
- decimal degrees
- Attribute:
- Attribute_Label:
- Longitude
- Attribute_Definition:
- The longitude where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- -88.21157
- Range_Domain_Maximum:
- -88.01
- Attribute_Units_of_Measure:
- decimal degrees
- Attribute:
- Attribute_Label:
- Bin
- Attribute_Definition:
- The part of the water column where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Enumerated_Domain:
- Enumerated_Domain_Value:
- S
- Enumerated_Domain_Value_Definition:
- Water sample was collected near the surface.
- Enumerated_Domain_Value_Definition_Source:
- DISL: Kiene Lab
- Enumerated_Domain_Value:
- M
- Enumerated_Domain_Value_Definition:
- Water sample was collected at mid-depth.
- Enumerated_Domain_Value_Definition_Source:
- DISL: Kiene Lab
- Enumerated_Domain_Value:
- B
- Enumerated_Domain_Value_Definition:
- Water sample was collected near the bottom.
- Enumerated_Domain_Value_Definition_Source:
- DISL: Kiene Lab
- Attribute:
- Attribute_Label:
- Bottom Depth
- Attribute_Definition:
- The total depth of the water column where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 3
- Range_Domain_Maximum:
- 35
- Attribute_Units_of_Measure:
- meter
- Attribute:
- Attribute_Label:
- Sample Depth
- Attribute_Definition:
- The depth where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 36
- Attribute_Units_of_Measure:
- meter
- Attribute:
- Attribute_Label:
- Temp.
- Attribute_Definition:
- Water temperature where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 8
- Range_Domain_Maximum:
- 35
- Attribute_Units_of_Measure:
- Celsius
- Attribute:
- Attribute_Label:
- Salinity
- Attribute_Definition:
- Salinity where the water sample was collected.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 35
- Attribute_Units_of_Measure:
- PSU
- Attribute:
- Attribute_Label:
- DMS (nM)
- Attribute_Definition:
- Dimethyl sulfide concentration.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 21
- Attribute_Units_of_Measure:
- nM
- Attribute:
- Attribute_Label:
- DMSPt (nM)
- Attribute_Definition:
- Total dimethylsulfoniopropionate (DMSP) concentration.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 160
- Attribute_Units_of_Measure:
- nM
- Attribute:
- Attribute_Label:
- BP pM L-1 h-1
- Attribute_Definition:
- Bacterial biomass production via leucine incorporation.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 1138
- Attribute_Units_of_Measure:
- pM / L h
- Attribute:
- Attribute_Label:
- Methane nM
- Attribute_Definition:
- Methane concentration.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 141
- Attribute_Units_of_Measure:
- nM
- Attribute:
- Attribute_Label:
- Winkler DO mg/L
- Attribute_Definition:
- Dissolved oxygen concentration as measured by Winkler titration.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 11
- Attribute_Units_of_Measure:
- mg/L
- Attribute:
- Attribute_Label:
- CTD Probe DO (mg/L)
- Attribute_Definition:
- Dissolved oxygen concentration as measured by SBE 43 dissolved oxygen sensor.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 12
- Attribute_Units_of_Measure:
- mg/L
- Attribute:
- Attribute_Label:
- CTD Probe DO % Sat
- Attribute_Definition:
- Dissolved oxygen percent saturation as measured by SBE 43 dissolved oxygen sensor.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- -23
- Range_Domain_Maximum:
- 140
- Attribute_Units_of_Measure:
- percent
- Attribute:
- Attribute_Label:
- DO Saturation value (mg/L)
- Attribute_Definition:
- Saturation DO concentration.
- Attribute_Definition_Source:
- DISL: Kiene Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 10
- Attribute_Units_of_Measure:
- mg/L
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- Metadata_Reference_Information:
- Metadata_Date:
- 20120921
- Metadata_Contact:
- Contact_Information:
- Contact_Organization_Primary:
- Contact_Organization:
- Dauphin Island Sea Lab
- Contact_Person:
- Data Management Specialist
- Contact_Position:
- Data Management Specialist
- Contact_Address:
- Address_Type:
- mailing and physical
- Address:
- 101 Bienville Blvd.
- City:
- Dauphin Island
- State_or_Province:
- Al
- Postal_Code:
- 36528
- Country:
- USA
- Contact_Voice_Telephone:
- 251-861-2141
- Contact_Electronic_Mail_Address:
- metadata@disl.org
- Hours_of_Service:
- 8-5:00 CST
- Contact_Instructions:
- Please email the metadata specialist for further information.
- Metadata_Standard_Name:
- FGDC Content Standard for Digital Geospatial Metadata
- Metadata_Standard_Version:
- FGDC-STD-001-1998
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