Changes
On July 27, 2022 at 2:33:03 PM UTC, 851c91e4-6343-45af-967a-20a33797707e:
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Added resource PRJNA630066_Assessment_protist_biodiversity_resilience.txt to Assessment of protist biodiversity and resilience of the benthic microbial assemblages in sub-tidal and intertidal from the Chandeleur Islands, Louisiana, 2015-06-29 to 2015-09-23
f | 1 | { | f | 1 | { |
2 | "author": "Alison Robertson", | 2 | "author": "Alison Robertson", | ||
3 | "author_email": "arobertson@disl.org", | 3 | "author_email": "arobertson@disl.org", | ||
4 | "creator_user_id": "851c91e4-6343-45af-967a-20a33797707e", | 4 | "creator_user_id": "851c91e4-6343-45af-967a-20a33797707e", | ||
5 | "extras": [ | 5 | "extras": [ | ||
6 | { | 6 | { | ||
7 | "key": "DOI", | 7 | "key": "DOI", | ||
8 | "value": "doi:10.7266/n7-vh1r-dy20" | 8 | "value": "doi:10.7266/n7-vh1r-dy20" | ||
9 | }, | 9 | }, | ||
10 | { | 10 | { | ||
11 | "key": "ISO.pointOfContact", | 11 | "key": "ISO.pointOfContact", | ||
12 | "value": "Alison Robertson <arobertson@disl.org>" | 12 | "value": "Alison Robertson <arobertson@disl.org>" | ||
13 | }, | 13 | }, | ||
14 | { | 14 | { | ||
15 | "key": "Place Keywords", | 15 | "key": "Place Keywords", | ||
16 | "value": "Chandeleur Islands, Louisiana, Gulf of Mexico" | 16 | "value": "Chandeleur Islands, Louisiana, Gulf of Mexico" | ||
17 | }, | 17 | }, | ||
18 | { | 18 | { | ||
19 | "key": "Temporal Begin", | 19 | "key": "Temporal Begin", | ||
20 | "value": "2015-06-29" | 20 | "value": "2015-06-29" | ||
21 | }, | 21 | }, | ||
22 | { | 22 | { | ||
23 | "key": "Temporal End", | 23 | "key": "Temporal End", | ||
24 | "value": "2015-09-23" | 24 | "value": "2015-09-23" | ||
25 | }, | 25 | }, | ||
26 | { | 26 | { | ||
27 | "key": "Theme Keywords", | 27 | "key": "Theme Keywords", | ||
28 | "value": "Deepwater Horizon oil spill, Bacteria, Archaea, | 28 | "value": "Deepwater Horizon oil spill, Bacteria, Archaea, | ||
29 | Eukarya, seagrass, coastal, sediment, 16S rRNA, 18S rRNA" | 29 | Eukarya, seagrass, coastal, sediment, 16S rRNA, 18S rRNA" | ||
30 | }, | 30 | }, | ||
31 | { | 31 | { | ||
32 | "key": "spatial", | 32 | "key": "spatial", | ||
33 | "value": "{ \"type\": \"Polygon\", \"coordinates\": [ [ [ | 33 | "value": "{ \"type\": \"Polygon\", \"coordinates\": [ [ [ | ||
34 | -88.84147, 29.93338 ], [ -88.82778, 29.93338 ], [ -88.82778, 29.86375 | 34 | -88.84147, 29.93338 ], [ -88.82778, 29.93338 ], [ -88.82778, 29.86375 | ||
35 | ], [ -88.84147, 29.86375 ], [ -88.84147, 29.93338 ] ] ] }" | 35 | ], [ -88.84147, 29.86375 ], [ -88.84147, 29.93338 ] ] ] }" | ||
36 | } | 36 | } | ||
37 | ], | 37 | ], | ||
38 | "groups": [], | 38 | "groups": [], | ||
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40 | "isopen": false, | 40 | "isopen": false, | ||
41 | "license_id": null, | 41 | "license_id": null, | ||
42 | "license_title": null, | 42 | "license_title": null, | ||
43 | "maintainer": "data@disl.org", | 43 | "maintainer": "data@disl.org", | ||
44 | "maintainer_email": "data@disl.org", | 44 | "maintainer_email": "data@disl.org", | ||
45 | "metadata_created": "2022-07-27T14:33:02.919471", | 45 | "metadata_created": "2022-07-27T14:33:02.919471", | ||
n | 46 | "metadata_modified": "2022-07-27T14:33:03.348630", | n | 46 | "metadata_modified": "2022-07-27T14:33:03.773510", |
47 | "name": | 47 | "name": | ||
48 | ty-and-resilience-of-the-benthic-microbial-assemblages-in7-vh1r-dy20", | 48 | ty-and-resilience-of-the-benthic-microbial-assemblages-in7-vh1r-dy20", | ||
49 | "notes": "### Abstract\nThis dataset is a raw sequence reads from | 49 | "notes": "### Abstract\nThis dataset is a raw sequence reads from | ||
50 | samples collected from Chandeleur Islands, Louisiana from 2015-06-29 | 50 | samples collected from Chandeleur Islands, Louisiana from 2015-06-29 | ||
51 | to 2015-09-23. Marine sediment microbial communities play a critical | 51 | to 2015-09-23. Marine sediment microbial communities play a critical | ||
52 | role in primary production, sediment oxygenation, key biogeochemical | 52 | role in primary production, sediment oxygenation, key biogeochemical | ||
53 | cycles, and provide a nutrient-rich food source for benthic | 53 | cycles, and provide a nutrient-rich food source for benthic | ||
54 | invertebrates. While the photosynthetic organisms drive productivity | 54 | invertebrates. While the photosynthetic organisms drive productivity | ||
55 | at the surface, protists and other non-photosynthetic organisms | 55 | at the surface, protists and other non-photosynthetic organisms | ||
56 | dominate metabolic processes including hydrocarbon degradation in | 56 | dominate metabolic processes including hydrocarbon degradation in | ||
57 | deeper sediments. In an effort to understand the response and recovery | 57 | deeper sediments. In an effort to understand the response and recovery | ||
58 | of benthic microbial assemblages in sediments we embarked on an | 58 | of benthic microbial assemblages in sediments we embarked on an | ||
59 | intensive field survey in the Chandeleur Islands, an area impacted by | 59 | intensive field survey in the Chandeleur Islands, an area impacted by | ||
60 | the Deepwater Horizon (DWH) spill. Field sites were selected | 60 | the Deepwater Horizon (DWH) spill. Field sites were selected | ||
61 | representing an oiling gradient based on NOAA Shoreline Cleanup and | 61 | representing an oiling gradient based on NOAA Shoreline Cleanup and | ||
62 | Assessment Technique (SCAT) surveys over a range of habitats, | 62 | Assessment Technique (SCAT) surveys over a range of habitats, | ||
63 | including salt marsh, un-vegetated sand, and seagrass meadows. | 63 | including salt marsh, un-vegetated sand, and seagrass meadows. | ||
64 | Triplicate sediment cores (0-30cm) were collected from each habitat | 64 | Triplicate sediment cores (0-30cm) were collected from each habitat | ||
65 | across the four field sites in June and September of 2015. Cores were | 65 | across the four field sites in June and September of 2015. Cores were | ||
66 | sectioned and sub-samples were taken for genomic DNA isolation. | 66 | sectioned and sub-samples were taken for genomic DNA isolation. | ||
67 | Microbenthic community abundance and biodiversity were investigated | 67 | Microbenthic community abundance and biodiversity were investigated | ||
68 | with Illumina MiSeq 2 x 250 bp technology for 108 sediment samples. | 68 | with Illumina MiSeq 2 x 250 bp technology for 108 sediment samples. | ||
69 | Research background and DNA sequence data can be accessed at the | 69 | Research background and DNA sequence data can be accessed at the | ||
70 | National Center for Biotechnology Information (NCBI) under accession | 70 | National Center for Biotechnology Information (NCBI) under accession | ||
71 | number PRJNA630066.\n\n### Purpose\nShared research information and | 71 | number PRJNA630066.\n\n### Purpose\nShared research information and | ||
72 | DNA sequences can be used as a reference by other researchers who | 72 | DNA sequences can be used as a reference by other researchers who | ||
73 | study oiling effects on microbial communities.\n\n**DOI: | 73 | study oiling effects on microbial communities.\n\n**DOI: | ||
74 | .7266/n7-vh1r-dy20](https://www.doi.org/10.7266/n7-vh1r-dy20)**\n\n### | 74 | .7266/n7-vh1r-dy20](https://www.doi.org/10.7266/n7-vh1r-dy20)**\n\n### | ||
75 | Suggested Citation\nAlison Robertson, Michael Parsons, Hidetoshi | 75 | Suggested Citation\nAlison Robertson, Michael Parsons, Hidetoshi | ||
76 | Urakawa. 2020. Assessment of protist biodiversity and resilience of | 76 | Urakawa. 2020. Assessment of protist biodiversity and resilience of | ||
77 | the benthic microbial assemblages in sub-tidal and intertidal from the | 77 | the benthic microbial assemblages in sub-tidal and intertidal from the | ||
78 | Chandeleur Islands, Louisiana, 2015-06-29 to 2015-09-23. Distributed | 78 | Chandeleur Islands, Louisiana, 2015-06-29 to 2015-09-23. Distributed | ||
79 | by: Gulf of Mexico Research Initiative Information and Data | 79 | by: Gulf of Mexico Research Initiative Information and Data | ||
80 | Cooperative (GRIIDC), Harte Research Institute, Texas A&M | 80 | Cooperative (GRIIDC), Harte Research Institute, Texas A&M | ||
81 | University\u2013Corpus Christi. doi:10.7266/n7-vh1r-dy20\n\n**Funded | 81 | University\u2013Corpus Christi. doi:10.7266/n7-vh1r-dy20\n\n**Funded | ||
82 | by:** Gulf of Mexico Research Initiative (GoMRI)\n\n**Funding cycle:** | 82 | by:** Gulf of Mexico Research Initiative (GoMRI)\n\n**Funding cycle:** | ||
83 | RFP-IV\n\n**Research group:** Alabama Center for Ecological Resilience | 83 | RFP-IV\n\n**Research group:** Alabama Center for Ecological Resilience | ||
84 | (ACER)\n\n", | 84 | (ACER)\n\n", | ||
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87 | "organization": { | 87 | "organization": { | ||
88 | "approval_status": "approved", | 88 | "approval_status": "approved", | ||
89 | "created": "2022-07-22T03:25:13.329710", | 89 | "created": "2022-07-22T03:25:13.329710", | ||
90 | "description": "The Dauphin Island Sea Lab organization.", | 90 | "description": "The Dauphin Island Sea Lab organization.", | ||
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94 | "name": "dauphin-island-sea-lab", | 94 | "name": "dauphin-island-sea-lab", | ||
95 | "state": "active", | 95 | "state": "active", | ||
96 | "title": "Dauphin Island Sea Lab", | 96 | "title": "Dauphin Island Sea Lab", | ||
97 | "type": "organization" | 97 | "type": "organization" | ||
98 | }, | 98 | }, | ||
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129 | "state": "active", | 154 | "state": "active", | ||
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131 | { | 156 | { | ||
132 | "display_name": "16S rRNA", | 157 | "display_name": "16S rRNA", | ||
133 | "id": "3d181df1-15a0-44d9-ac5a-f3fd1f1a8a01", | 158 | "id": "3d181df1-15a0-44d9-ac5a-f3fd1f1a8a01", | ||
134 | "name": "16S rRNA", | 159 | "name": "16S rRNA", | ||
135 | "state": "active", | 160 | "state": "active", | ||
136 | "vocabulary_id": null | 161 | "vocabulary_id": null | ||
137 | }, | 162 | }, | ||
138 | { | 163 | { | ||
139 | "display_name": "18S rRNA", | 164 | "display_name": "18S rRNA", | ||
140 | "id": "673fa747-2ef2-47fc-9453-45b1690b44ad", | 165 | "id": "673fa747-2ef2-47fc-9453-45b1690b44ad", | ||
141 | "name": "18S rRNA", | 166 | "name": "18S rRNA", | ||
142 | "state": "active", | 167 | "state": "active", | ||
143 | "vocabulary_id": null | 168 | "vocabulary_id": null | ||
144 | }, | 169 | }, | ||
145 | { | 170 | { | ||
146 | "display_name": "Archaea", | 171 | "display_name": "Archaea", | ||
147 | "id": "968335f5-b446-4953-a27b-092f8c00e26e", | 172 | "id": "968335f5-b446-4953-a27b-092f8c00e26e", | ||
148 | "name": "Archaea", | 173 | "name": "Archaea", | ||
149 | "state": "active", | 174 | "state": "active", | ||
150 | "vocabulary_id": null | 175 | "vocabulary_id": null | ||
151 | }, | 176 | }, | ||
152 | { | 177 | { | ||
153 | "display_name": "Bacteria", | 178 | "display_name": "Bacteria", | ||
154 | "id": "274340f5-262a-4fe1-b1fe-ccd36ec39085", | 179 | "id": "274340f5-262a-4fe1-b1fe-ccd36ec39085", | ||
155 | "name": "Bacteria", | 180 | "name": "Bacteria", | ||
156 | "state": "active", | 181 | "state": "active", | ||
157 | "vocabulary_id": null | 182 | "vocabulary_id": null | ||
158 | }, | 183 | }, | ||
159 | { | 184 | { | ||
160 | "display_name": "Chandeleur Islands", | 185 | "display_name": "Chandeleur Islands", | ||
161 | "id": "1187d400-d9de-4166-aca2-2e591ecf8c65", | 186 | "id": "1187d400-d9de-4166-aca2-2e591ecf8c65", | ||
162 | "name": "Chandeleur Islands", | 187 | "name": "Chandeleur Islands", | ||
163 | "state": "active", | 188 | "state": "active", | ||
164 | "vocabulary_id": null | 189 | "vocabulary_id": null | ||
165 | }, | 190 | }, | ||
166 | { | 191 | { | ||
167 | "display_name": "Deepwater Horizon oil spill", | 192 | "display_name": "Deepwater Horizon oil spill", | ||
168 | "id": "3dba9434-7ecc-4aa5-a9f4-df31c3311b85", | 193 | "id": "3dba9434-7ecc-4aa5-a9f4-df31c3311b85", | ||
169 | "name": "Deepwater Horizon oil spill", | 194 | "name": "Deepwater Horizon oil spill", | ||
170 | "state": "active", | 195 | "state": "active", | ||
171 | "vocabulary_id": null | 196 | "vocabulary_id": null | ||
172 | }, | 197 | }, | ||
173 | { | 198 | { | ||
174 | "display_name": "Eukarya", | 199 | "display_name": "Eukarya", | ||
175 | "id": "5a302a9d-2ca9-4c4f-bdb3-5f23c95eae17", | 200 | "id": "5a302a9d-2ca9-4c4f-bdb3-5f23c95eae17", | ||
176 | "name": "Eukarya", | 201 | "name": "Eukarya", | ||
177 | "state": "active", | 202 | "state": "active", | ||
178 | "vocabulary_id": null | 203 | "vocabulary_id": null | ||
179 | }, | 204 | }, | ||
180 | { | 205 | { | ||
181 | "display_name": "Gulf of Mexico", | 206 | "display_name": "Gulf of Mexico", | ||
182 | "id": "26b2a6bc-e01b-4c22-9d3f-5bbcd148cc4f", | 207 | "id": "26b2a6bc-e01b-4c22-9d3f-5bbcd148cc4f", | ||
183 | "name": "Gulf of Mexico", | 208 | "name": "Gulf of Mexico", | ||
184 | "state": "active", | 209 | "state": "active", | ||
185 | "vocabulary_id": null | 210 | "vocabulary_id": null | ||
186 | }, | 211 | }, | ||
187 | { | 212 | { | ||
188 | "display_name": "Louisiana", | 213 | "display_name": "Louisiana", | ||
189 | "id": "d7699c97-9202-4624-bb36-265d2a628a57", | 214 | "id": "d7699c97-9202-4624-bb36-265d2a628a57", | ||
190 | "name": "Louisiana", | 215 | "name": "Louisiana", | ||
191 | "state": "active", | 216 | "state": "active", | ||
192 | "vocabulary_id": null | 217 | "vocabulary_id": null | ||
193 | }, | 218 | }, | ||
194 | { | 219 | { | ||
195 | "display_name": "coastal", | 220 | "display_name": "coastal", | ||
196 | "id": "ef936b5d-7d1f-40d2-b1e2-4608120a1274", | 221 | "id": "ef936b5d-7d1f-40d2-b1e2-4608120a1274", | ||
197 | "name": "coastal", | 222 | "name": "coastal", | ||
198 | "state": "active", | 223 | "state": "active", | ||
199 | "vocabulary_id": null | 224 | "vocabulary_id": null | ||
200 | }, | 225 | }, | ||
201 | { | 226 | { | ||
202 | "display_name": "seagrass", | 227 | "display_name": "seagrass", | ||
203 | "id": "5824ced6-3e9f-41c8-abac-b98bcff0e203", | 228 | "id": "5824ced6-3e9f-41c8-abac-b98bcff0e203", | ||
204 | "name": "seagrass", | 229 | "name": "seagrass", | ||
205 | "state": "active", | 230 | "state": "active", | ||
206 | "vocabulary_id": null | 231 | "vocabulary_id": null | ||
207 | }, | 232 | }, | ||
208 | { | 233 | { | ||
209 | "display_name": "sediment", | 234 | "display_name": "sediment", | ||
210 | "id": "d786ab28-d532-4342-a122-d99565e6d8ce", | 235 | "id": "d786ab28-d532-4342-a122-d99565e6d8ce", | ||
211 | "name": "sediment", | 236 | "name": "sediment", | ||
212 | "state": "active", | 237 | "state": "active", | ||
213 | "vocabulary_id": null | 238 | "vocabulary_id": null | ||
214 | } | 239 | } | ||
215 | ], | 240 | ], | ||
216 | "title": "Assessment of protist biodiversity and resilience of the | 241 | "title": "Assessment of protist biodiversity and resilience of the | ||
217 | benthic microbial assemblages in sub-tidal and intertidal from the | 242 | benthic microbial assemblages in sub-tidal and intertidal from the | ||
218 | Chandeleur Islands, Louisiana, 2015-06-29 to 2015-09-23", | 243 | Chandeleur Islands, Louisiana, 2015-06-29 to 2015-09-23", | ||
219 | "type": "dataset", | 244 | "type": "dataset", | ||
220 | "url": null, | 245 | "url": null, | ||
221 | "version": null | 246 | "version": null | ||
222 | } | 247 | } |